![]() However, the current Förster resonance energy transfer sensors are limited in utility because their binding affinity lies outside the physiological range of ABA levels or expression of the sensors interferes with ABA signaling. Förster resonance energy transfer-based ABA sensors have been developed to study the spatial dynamics of ABA accumulation in root cells ( Jones et al., 2014 Waadt et al., 2014). Reporters that detect the presence and activity of ABA at each step of regulation are needed to study how ABA signaling controls complex physiological responses across the various tissues and organ systems of the plant during stress. These stresses cause a reduction in the availability of water to the plant, leading to the induction of ABA biosynthesis and subsequent perception by the ABA signaling pathway ( Seo and Koshiba, 2002). ![]() This work establishes a tool to study the spatial pattern of ABA-mediated transcriptional regulation and a repertoire of TF-ABRE interactions that contribute to the developmental and environmental control of gene expression in roots.Ībscisic acid (ABA) is a plant hormone, widely involved in different biological processes, including plant growth ( Humplík et al., 2017), stomatal conductance ( Negin and Moshelion, 2016), lateral root development ( Duan et al., 2013), and seed dormancy ( Leprince et al., 2017), as well as response to water-associated environmental stresses, such as water deficit and osmotic and salt stress ( Seo and Koshiba, 2002 Munemasa et al., 2015 Sah et al., 2016). We show that the WUSCHEL RELATED HOMEOBOX5 and NAC DOMAIN PROTEIN13 TFs regulate QC/SCN expression of the ABRE reporter, which highlights the convergence of developmental and DNA-damage signaling pathways onto this cis-element in the absence of water stress. Our analysis also revealed ABA-independent activity of the ABRE-reporter under nonstress conditions, with expression being enriched at the quiescent center and stem cell niche. Genome-scale yeast one-hybrid screens complemented these approaches and revealed how promoter sequence and architecture affect the recruitment of diverse transcription factors (TFs) to the ABRE. Here, we describe the construction and evaluation of an ABSCISIC ACID RESPONSIVE ELEMENT (ABRE)-based synthetic promoter reporter that reveals the transcriptional response of tissues to different levels of exogenous ABA and stresses. Although ABA signaling is known to function in specific tissues to regulate root growth, little is understood regarding the spatial pattern of ABA-mediated transcriptional regulation. The water stress-associated hormone abscisic acid (ABA) acts through a well-defined signal transduction cascade to mediate downstream transcriptional events important for acclimation to stress.
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